Fig. 3

In a model of inflammation that utilized LPS-stimulated monocytes, the early and immediate addition of ATP decreased the levels of CCL18 (A; 1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 10 µM ATP, p < 0.05; 1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 100 µM ATP, p < 0.05). In contrast, the early addition of ATP to LPS-stimulated monocytes did not alter the levels of IL-1β (B; 1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 10 µM ATP n.s.; 1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 100 µM ATP n.s.). Similarly, delayed incubation with ATP (C) did not affect the levels of CCL18 in LPS-stimulated monocytes (1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 10 µM ATP n.s.; 1.0 µg/mL LPS vs. 1.0 µg/mL LPS + 100 µM