Fig. 2

Migratory conventional dendritic cells in mesenteric lymph nodes express an activated phenotype upon live soil exposure. Spleen and mesenteric lymph nodes were collected at day 21 and myeloid cell compartments were analyzed using flow cytometry. Conventional dendritic cells 1 (cDC1) and 2 (cDC2) were defined as XCR1⁺SIRPα⁻ or XCR1⁻SIRPα⁺, respectively, and quantified from the from the CD11c^Int/HiMHCII^Hi population (A). The percentage of cDC1 and cDC2 showed no differences between mouse groups. (B) CD80 expression on cDC1 and cDC2 was quantified using median fluorescence intensity as a measure for activation. Data are shown as scatter plots with mean and 95% CI (n = 8 per group, from two independent exposure experiments). Each symbol represents an individual mouse, lines indicate the mean. The data was analyzed with One-way ANOVA with Tukey´s multiple comparison test, *p < 0.05