Fig. 3

Exposure to autoclaved soil downregulates IgM in B1 cells from spleen while live soil upregulates CD8⁺T cells in mesenteric lymph nodes. Spleen and mesenteric lymph nodes were collected at day 21 and lymphoid cell compartments were analyzed using flow cytometry. (A) T cells (CD3⁺), helper T cells (CD3⁺ CD4⁺), cytotoxic T cells (CD3⁺ CD8⁺) and B cells (CD19⁺) in the mLN. (B) The percentage of CD69 + effector cytotoxic T cells (CD8⁺ CD44⁺ CD62L⁻) in the mLN. (C) CD3⁺, CD4⁺, CD8⁺ and CD19⁺ cells in spleen. (D) B1 cells (CD19⁺ B220⁻) and IgM expression on B1 cells in spleen. MFI was measured from total B1 population. (E) B2 cells (CD19⁺ B220⁺) and IgM expression on B2 cells in spleen. MFI was measured from total B2 population. Data are shown as scatter plots with mean and 95% CI (n = 8 per group, from two independent exposure experiments). Each symbol represents an individual mouse, lines indicate the mean. The data was analyzed with One-way ANOVA with Tukey´s multiple comparison test, *p < 0.05