Fig. 5

In colon, exposure to autoclaved soil upregulatesinos, RorγtandFoxp3expression while live soil exposure downregulatesRorγt. In mesenteric lymph nodes, live soil exposure downregulatesGata3andFoxp3expression. Gene expression changes in different organs after no treatment, autoclaved or live soil exposure. RNA was extracted from the colon, mLN, lung or small intestine. Gene expression was measured using qPCR. (A) Macrophage activation associated genes (Arg1 and inos) and T cell lineage associated genes (Tbet, Gata3, Rorγt, and Foxp3), in the colon. (B) T cell lineage-associated genes (Tbet, Gata3, Rorγt, and Foxp3) were measured in the mLN. (C) Macrophage activation associated genes (Arg1 and inos), Il21 cytokine and Foxp3 expression were measured in the lung. (D) Macrophage activation associated genes (Arg1 and inos) and T cell lineage associated genes (Tbet, Gata3, Rorγt, and Foxp3), in the small intestine. Values are shown as scatter plots with bar as mean and SEM (n = 8 per group, from two independent exposure experiments). Fold changes were calculated using control group mean and all data points were compared accordingly, including the control group. The data were analyzed with One-way ANOVA test with Tukey´s multiple comparison test, *p < 0.05, **p < 0.01