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Fig. 2 | BMC Immunology

Fig. 2

From: The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through membrane immunoassay device

Fig. 2

The selection of appropriate detection and capture antibodies using a sandwich assay. a Reactivity and specificity of the candidate mAbs. Forty candidate mAbs were tested with either Wuhan CoV-2-NP or CoV-NP to evaluate cross-reactivity by ELISA. Data are expressed as the mean of two measurements. b Pairwise analysis of mAbs against Wuhan CoV-2-NP. Sandwich assay was used for pairwise coupling of thirteen capture mAbs against sixteen detection mAbs. The numbers inside the grid are the luminescence activities. The heatmap represents the performance of antibody pair. Red, > 80,000; orange, 80,000–60000; green, 59,999–40,000; blue, 39,999–20,000; gray, not determined. Data are expressed as the mean of two measurements. The highest luminescence was considered as the optimal mAb set

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BMC Immunology

ISSN: 1471-2172

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