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Fig. 5 | BMC Immunology

Fig. 5

From: The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through membrane immunoassay device

Fig. 5

Determination of the technical sensitivity of CoV-2-POCube. a A schematic of CoV-2-POCube. Immunocomplex formation in the test sample followed by the sample loaded into a reaction vessel (I). Immunocomplex trapping on an anti-biotin antibody-coated membrane followed by washing to remove contaminants (II). Luminescence signal measurement (III). Reaction time requires 10 min for (I) and 5 min for (II) ~ (III), totaling 15 min. b The calibration curve of CoV-2-POCube for CoV-2-NP detection. Serially diluted recombinant CoV-2-NP of Delta or Omicron variant were subjected to CoV-2-POCube analysis. c COV-2-POCube signal intensity of serially diluted heat-inactivated Wuhan SARS-CoV-2 particles. Each dilution was analyzed for CoV-2-NP using a COV-2-POCube. d Correlation between COV-2-POCube signal and CoV-2-NP concentrations. COV-2-POCube signal intensity of serially dilute Delta CoV-2-NPs are shown. All data are presented as the mean ± SD of three replicates

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BMC Immunology

ISSN: 1471-2172

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