Fig. 1

Bcl-3 depletion decreased the energy metabolism of Jurkat cells. Cells (5 × 10⁵) were seeded in 96-well XF Seahorse incubation plates as indicated in the protocol. Cells were cultured at 37 °C in XF base medium (pH 7.4), and FCCP (1 µM), oligomycin (1 µM), Rot (0.5 µM), glucose (10 mM) and 2-DG (50 mM) were added sequentially into the plates at specific time points following the manufacturer’s guidelines. The oxygen consumption rate (A, B) and extracellular acidification rate (C, D) of Jurkat and Bcl-3^−/− Jurkat cells were determined using an XF24e extracellular flux analyzer