Fig. 2

Phenotypic characteristics of mo-DCs. At day 0 (Day 0), CD14⁺ monocytes from HD group and CHB group were isolated and incubated with recombinant human GM-CSF plus IL-4 for 6 days (Day 6). Then the cells were continued to be cultured with unstimulated condition (control group), LPS, supernatant of HepG2.2.15 cells and supernatant of HepG2 cells until day 10 (Day 10) respectively. The cells were stained for CD11c, (human leukocyte antigen- DR )HLA-DR and CD80/CD86/CD83. (A) The living cells were gated initially on CD11c⁺ (upper panel) and then on CD11c⁺HLA-DR⁺ cells (lower three panels). Flow cytometry histograms showing the fluorescence intensities of HLA-DR, CD80, CD86, CD83 of each time point of cells from HD group (red) and CHB group (green) and isotype control (yellow). Numbers from three independent analyses indicated mean fluorescence intensities (MFI). (B) The MFI of HLA-DR in CD11c⁺ cells and CD80/CD86/CD83 in CD11c⁺HLA-DR⁺ cells were compared between each group at each time point. Means and standard deviations of cell numbers from three independent analyses are shown *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001, NS, not significant (P > 0.05)