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Fig. 3 | BMC Immunology

Fig. 3

From: Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection

Fig. 3

Cytokines secretion of mo-DCs. CD14+ monocytes from HD group and CHB group were isolated and incubated with recombinant human GM-CSF plus IL-4 for 6 days. Then the cells were continued to be cultured with unstimulated condition (control group), LPS, supernatant of HepG2.2.15 cells and supernatant of HepG2 cells until day 10 (Day 10) respectively. Then the concentration of tumour necrosis factor-α(TNF-α), IL-10 and IL-12 p70 in the supernatant of cultured cells were detected by enzyme-linked immunosorbent assay (ELISA). The concentration of TNF-α (left panel), IL-10 (middle panel) and IL-12 p70 (right panel), in the supernatant of cultured DCs from indicated group were compared. Means and standard errors of the concentration are shown. Representative results of three independent experiments are shown. *, P < 0.05, **,P < 0.01, ***, P < 0.001, ****, P < 0.0001. NS, not significant

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BMC Immunology

ISSN: 1471-2172

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