Fig. 4
Expression of autophagy-related proteins in DCs. Two-color immunofluorescence analysis of DCs from HD (upper panel) and patients with CHB(lower panel), which cultured with control medium, LPS, supernatant of HepG2.2.15 cells, supernatant of HepG2 cells respectively. Cultured cells were stained with anti-LC3 antibody (green) and PI (red). Representative images from three independent experiments are shown. (B-C) Western blot analysis of associated protein 1 light chain (LC3 protein) (B) and autophagy-related protein-5(Atg5) protein (C) expression in cultured DCs from HD and patients with CHB. Shown are the representative data of three independent experiments. Means and standard deviations of relative grey-scale intensity of LC3 (B, right graph) and Atg5 (C, right graph) expression in indicated group are shown. *, P < 0.05, **, P < 0.01, NS, not significant (P > 0.05)