Fig. 7

MENK upregulated effector memory CD8⁺ T cells of mice infected with influenza virus. A Representative dot plots of CD8⁺CD44^hi T cells in BALF, lung, and LN of mice on day 10 p.i.. Representative dot plots of CD62L^loIFN-γ⁺ cells in CD8⁺CD44^hi T cells in BALF (B), lung (C), and LN (D) of mice on day 10 p.i. Cells were stimulated with absence or presence of influenza NP366-374 or PA224-233 peptides and stained as described in Materials and Methods. E Panel presenting the proportion of NP/PA-effector memory CD8⁺ T cells (CD8⁺CD44^hiCD62L^lo IFN-γ⁺) stimulated with NP or PA in BALF, lung, and LN. The data represent the mean ± SD of three independent experiments (each performed with n = 6 mice). ^★p < 0.05, ^★★p < 0.01 versus the PR8 group