Fig. 2
The images shown are representative of sections obtained from 5 animals per group. (A). Histology of lung in a healthy guinea pig. (B,C). M.tb infected and M.tb infected Xpro-treated guinea pigs 4th week post-infection. (D, E). M.tb infected, and M.tb infected Xpro-treated guinea pigs, 8th week post-infection. Solid granulomas exhibited caseous necrotic lesions with no cellular regions in the M.tb infected guinea pigs. Granulomas in Xpro-treated guinea pigs showed reduced necrosis and open-air spaces. (F) (G). Lung pathology in animals receiving anti-TB drugs (Isoniazid (H) and rifampicin (R)) adjunctive Xpro-1595 (H plus Xpro-1595) appeared to resolve with more open-air spaces than those receiving standard treatment (RH alone) respectively. Experiments were performed on triplicate sections collected from n = 5 guinea pigs in each group. All data in each figure is from one biological repeat. M.tb output was expressed as mean log 10 CFU/ml ± SE (n = 5). One-way ANOVA test was used to compare the differences between treated and untreated cultures with statistical significance indicated as *** (p < 0.001)