Fig. 7

(A). CD4 + T cell expression in healthy guinea pig 7B,7 C. CD4 + T cell expression was significantly elevated in M.tb infected guinea pigs during the 4th week (cell count mean value/unit area = 188.54) as compared to that in M.tb infected Xpro-1595 treated guinea pigs (cell count represent value/unit area- 160.63) respectively; the results were obtained using triplicate sections of tissue followed by analysis using IHC tool plugin in ImageJ software. (D and E). CD4 + T cell expression was significantly reduced in both M.tb infected guinea pigs, and M.tb infected Xpro-1595 treated guinea pigs during the 8th week compared to that in the 4th week; the results were obtained using IHC tool plugin in ImageJ software. (F). The Xpro-1595 treated group alone (G, H). During the treatment with RH + Xpro-1595, we found a reduced CD4 + T cell count compared with RH treatment group providing the evidence of an early diminish in bacteria after the chemotherapy. A2, B2, C2, D2, E2, F2, G2 and H2 are staining detection images identified by ImageJ that distinguishes DAB and HE stain. The experiments were conducted in triplicate on tissues collected from five guinea pigs in each group. Each figure displays data from a single biological replicate