Fig. 2

Sec1 mediates systemic and local inflammation in mice and apoptosis of intestinal epithelial cells. (A) ELISA was used to determine the concentrations of IL-1β, IL-6 and TNF-α in the serum of mice. (B, C) Qualitative and quantitative analyses of Th17 cells (B) and T reg cells (C) in spleen lymphocytes of WT, WT + DSS, Sec1^−/− + DSS mice using flow cytometry. Data were expressed as the mean ± standard deviation. n = 3 biological repeats. (D) TUNEL was used to evaluate the apoptosis of colonic epithelial cells in the WT group, WT + DSS group and Sec1^−/− + DSS group; apoptotic cells showed red fluorescence. The experiment was carried out in three biological repeats. Scale bars = 40 μm