Fig. 3

Sec1 was associated with the down-regulation of inflammatory cytokines, the promotion of epithelial cell proliferation and the inhibition of apoptosis. (A) IL-1β, IL-6, TNF-α concentrations in the supernatants of CT26.WT, CMT93, CT26.WT Sec1-siRNA and CMT93 Sec1-siRNA cells. (B) RT-qPCR was used to determine the expression of genes encoding IL-1β, IL-6 and TNF-α. Data were expressed as the mean ± SEM. n = 3. *p < 0.05, ***p < 0.001. (C) Cell proliferation. Cell growths of CT 26.WT, CMT93, CT26.WT Sec1-siRNA and CMT93 Sec1-siRNA cells at 24 h, 48 and 72 h were determined using a spectrophotometer, and ratios of siRNA treated cells to untreated cells were calculated. NC, untreated negative control. (D) Apoptosis of siRNA treated and untreated cells. Representative flow cytometry plots can be found in supplementary Fig. 1. (E) Cell cycle of siRNA treated and untreated cells. NC, untreated negative control