Fig. 4

(A) Dr5 gene expression in colonic epithelial cells from WT, WT + DSS, Sec1-/- + DSS mice were assessed by QT-qPCR. (B) The syntheses of DR5 protein in colonic epithelial cells of three groups were confirmed by western-blot assays. (C) Detection of Sec1 and DR5 proteins in mouse colon epithelial cells using immunofluorescence assays. Scale bars = 100 μm. (D) Dr5 gene expression in CT26.WT, CMT93, CT26.WT Sec1-siRNA, CMT93 Sec1-siRNA cells were determined by QT-qPCR. (E) Expression of DR5, and apoptosis-related proteins cleaved Caspase-3, Caspase-8, Caspase-9, Bax and BCL-2 in different groups were determined by western-blot assays. Data were expressed as the mean ± SEM. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. To be noted, membranes with transferred proteins were cut prior to antibody hybridization