Fig. 1
From: Increased infiltration of CD4+ T cell in the complement deficient lymphedema model
Postoperative upregulated C3 expression and complement activation in the tail lymphedema. A The time course of the increased ratio of tail swelling volume after the surgery in wild-type C57BL6/J mice (n=3). Data are shown as the means ± standard error. B Relative expression levels of C3 mRNA in the tail lymphedema tissue (edema) compared with non-operated controls (Ctrl) (n = 3/group). The expression levels were normalized to those of 18s rRNA. Data are shown as the means ± standard deviation; *P<0.05. C Representative image of C3 protein (Green) around lymphatic vessels (LYVE1, Magenta) on POD21. Nuclei were stained with DAPI (Blue). Scale bars = 100 µm. D Representative image of C4 protein (Green) around lymphatic vessels (LYVE1, Magenta) at an adjacent section of (C). Nuclei were stained with DAPI (Blue). Scale bars = 100 µm