Fig. 3

Infiltration of macrophages in lymphedema tissues on POD21. A Detection of F4/80⁺ macrophages (Green) in lymphedema tissues of wild-type (WT) and C3 KO mice. B Comparison of numbers of F4/80⁺ macrophages per field (8 fields/mouse) of wild-type (WT) and C3 KO mice in lymphedema tissues (n = 5/group). Horizontal bars indicate the averages. C Detection of F4/80⁺ macrophages (Green) in lymphedema tissues of wild-type (WT) and C5 KO mice. D Comparison of numbers of F4/80⁺ macrophages per field (8 fields/mouse) of wild-type (WT) (n = 4) and C5 KO (n = 5) mice in lymphedema tissues (n = 4/group). Horizontal bars indicate the averages. Representative images are shown in (A) and (C). Nuclei were stained with DAPI (Blue). Scale bars = 100 μm