Fig. 4

Infiltration of CD4⁺ T cells in lymphedema tissues on POD21.  A Detection of CD4⁺ T cells (Green) and lymphatic vessels (Magenta) in lymphedema tissues of WT and C3 KO mice. B Comparison of numbers of CD4⁺ T cells per field (8 fields/mouse) of WT and C3 KO mice in lymphedema tissues (n = 8/group). Horizontal bars indicate the averages; * P  < 0.05. C Detection of CD4⁺ T cells (Green) and lymphatic vessels (Magenta) in lymphedema tissues of WT and C5 KO mice. D Comparison of numbers of CD4⁺ T cells per field (8 fields/mouse) of WT (n = 5) and C5 KO (n = 6) mice in lymphedema tissues (n = 8/group). Horizontal bars indicate the averages.  Representative images are shown in (A) and (C). Nuclei were stained with DAPI (Blue). Scale bars = 100 μm