Fig. 8

Single-cell RNA sequencing. A The total sequencing diagram showed that, following dimensionality reduction, the cells were categorized into nine distinct groups. B Among the identified cell lines, T cells were represented by 1, 2, 6, and 8, Macrophages by 3 and 5, NK cells by 4, B cells by 7, and Platelets by 9. C In the dotplot, the gene name was plotted on the abscissa, while the cell group number was plotted on the ordinate. The size of the dot corresponded to the proportion of cells expressing the gene within that particular cell group. Additionally, a color gradient ranging from blue to red indicated varying levels of gene expression, with red indicating higher expression. The findings of this study indicate that GNLY, GZMB, and PRF1 were predominantly localized within cell lines 2 and 4, while RASGRP1 exhibited predominant localization within cell lines 1, 2, 4, 6, and 8. D-G, A dot in a feature plot represented an individual cell, with the color gradient ranging from blue to red, signifying higher gene expression levels within the cell. These results further demonstrate that GNLY, GZMB, PRF1, and RASGRP1 exhibit high expression levels, specifically within T cells and NK cells