Fig. 2
Activation and expression profiles of murine DCs and MΦs against BCG infection. (A) Detection of infection rate of LN MΦs against rBCG-GFP. C57BL/6 mice (n = 5) were s.c injected with 1 × 108 CFU rBCG-GFP, LN cells were harvested at different time points, and MΦs were sorted and analysed for the presence of rBCG-GFP. The results are representative of three independent experiments. (B) CFU detection of LN MΦs against BCG. Mice (n = 5) were s.c injected with 1 × 108 CFU BCG; BCG in MΦs was quantified in CFUs by culturing on Middlebrook 7H10 agar. (C) Transcriptome analysis of murine LN DCs and MΦs against BCG infection. C57BL/6 mice were s.c injected with 1 × 108 CFU BCG cells in PBS. Mice were sacrificed at 12 and 48 h, and inguinal LNs single-cell suspensions were prepared, DCs and MΦs were sorted with an autoMACS separator. The RT2 profiler PCR array about antigen presentation pathway associated genes was carried out by QIAGEN, where RNA isolation and quality control were completed. The matrix numbers 1–12 and A-G in heat maps correspond to distinct genes about antigen presentation cells. The results are presented as means ± SEM. Statistical significance was determined using Student’s t-test (*P < 0.05, **P < 0.01)