Fig. 1

Th2 cells reduce HFD-induced obesity of mice. Eight-week-old CD3ε^─/─ male mice were intravenous injection of Th2 cells isolated from LAT^Y136F mice or PBS in the presence of High-fat diet (HFD) for 16 weeks respectively. A The body weight changes during the experiments were monitored. (n = 5). B Photographs of representative mice fed on HFD. Representative appearance of epididymal adipose tissue (EAT), subcutaneous adipose tissue (SAT), perirenal adipose tissue (PRAT), brown adipose tissue (BAT) (C) and ratio of tissue weight to body weight (D). E Representative H&E staining of EAT, SAT, PRAT and BAT. Scale bars = 50 µm. F The protein expression of long-chain acyl-CoA synthetase (ACSL), FAS (fatty acid synthase), Acetyl-CoA Carboxylase (ACC) and Adipose differentiation-related protein (ADRP) was determined by western blot analysis. G The serum concentrations of triglyceride (TG), cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were evaluated at the indicated time points. *P < 0.05, **P < 0.01, NS, not significant. Data from one representative experiment of three independent experiments are presented. Cropped images were adopted and full-length blots/gels are presented in Supplementary information