Fig. 6
From: Polarized Th2 cells attenuate high-fat-diet induced obesity through the suppression of lipogenesis
Th2 cells inhibit adipocyte differentiation. 3T3-L1 preadipocyte cells were induced into adipocytes firstly and then co-cultured with different number of Th2 cells for 24 h. A Morphology of fat cells were recorded in bright field. B Adipocytes differentiation was determined by staining with oil red with different numbers of Th2 cells. Scale bar = 50 μm. C The mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1c), CCAAT/enhancer-binding proteins (C/EBP) and fatty acid-binding protein 4 (FABP4) in adipocytes were determined by qRT-PCR analysis. D The protein level of acetyl-CoA synthetase (AceCS1), long-chain acyl-CoA synthetase (ACSL), FAS (fatty acid synthase), phosphorylated Acetyl-CoA Carboxylase (ACC) and Adipose differentiation-related protein (ADRP) was evaluated by immunoblotting analysis. Cropped images were adopted and full-length blots/gels are presented in Supplementary information