Fig. 7

Th2 cells inhibit adipocyte differentiation through oxphos. A 3T3-L1 preadipocyte cells were induced into adipocytes firstly and then co-cultured with different number of Th2 cells for 24 h, then the protein levels of PDH and OXPHOS were detected by immunoblotting. B-D 3T3-L1 preadipocyte cells were induced into adipocytes firstly and then co-cultured with different number of Th2 cells with rotenone for 24 h. B The mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1c), CCAAT/enhancer-binding proteins (C/EBP) and fatty acid-binding protein 4 (FABP4) in adipocytes were determined by qRT-PCR analysis. C The protein level of acetyl-CoA synthetase (AceCS1), long-chain acyl-CoA synthetase (ACSL), FAS (fatty acid synthase), and Adipose differentiation-related protein (ADRP) was evaluated by immunoblotting analysis. D Adipocytes were determined by staining with oil red. Scale bar = 50 μm. Abbreviations: high-fat-diet (HFD), epididymal adipose tissue (EAT), subcutaneous adipose tissue (SAT), perirenal adipose tissue (PRAT), brown adipose tissue (BAT), long-chain acyl-CoA synthetase (ACSL), FAS (fatty acid synthase), Acetyl-CoA Carboxylase (ACC), triglyceride (TG), cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), hematoxylin–eosin staining ( H&E staining), acetyl-CoA synthetase (AceCS1), ATP-Citrate Lyase (ACL), sterol regulatory element binding protein 1 (SREBP1c), fatty acid-binding protein 4 (FABP4), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins (C/EBP), intraperitoneal glucose tolerance (GTT), insulin tolerance test (ITT)