Fig. 4

Increased intestine IgA responses to the H. pylori antigen by alginate/pCI-neo-UreH. Vaccines were Orally administered to mice. ELISA measured specific IgA antibody titers in fecal pellet supernatants before each immunization cycle and two weeks after the final immunization round. A) For each group of mice that received the vaccine, the graph line depicts the change in IgA titers over time. Animals were infused to extract the blood from the organs two weeks after the second immunization round, and tissue was recovered. IgA titers specific to H. pylori were assessed in extracts from the (B) stomach, (C) jejunum, and (D) ileum. Data from three separate trials with an average of five mice per group and experiment. *p < 0.05, **p < 0.01, ***p < 0.00