Fig. 5

Alginate/pCI-neo-UreH vaccinations were administered intragastrically to WT, IL-17R-/-mice to elicit intestinal, but not systemic, Th1-type responses. Two weeks after the prior vaccination, cells were isolated from the spleen and mesenteric lymph nodes (MLN) and restimulated ex vivo with purified MP305 for 72 h. Supernatants were obtained and subjected to an IgA test from the fecal pellet extract (A), jejunum (B), ileum (C), and colon (D). Results from two distinct investigations, each comprising five animals in each group. *p < 0.05, **p < 0.01; ***p < 0.001