Fig. 3

Gating strategy of BAT 2. Example of an analysis with yellow jacket venom (YJV). A CD45-Krome Orange vs. SSC-A dot plot was created. A region was drawn to encompass the CD45^pos leukocytes. A plot 2 (CD3-PC7 vs. CD294 (CRTH2)-Alexa Fluor 647) was created and the leukocyte gate was applied to the plot. A plot 3 (CD63-PacBlue vs. CD203c-PE) was created and the CRTH2^pos CD3^neg cells gate was applied to this plot. A quadrant gate was selected and applied to this plot. Activated basophils result in a CD63^pos/CD203c^pos basophil population in the upper right quadrant