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Fig. 3 | BMC Immunology

Fig. 3

From: Dendritic cells under allergic condition enhance the activation of pruritogen-responsive neurons via inducing itch receptors in a co-culture study

Fig. 3

An increase of MHCII+ cells in DRG under allergic contact dermatitis condition. (A) Zamboni fixed sections of DRG were stained with MHC II for DCs and NeuN for neurons. The images were taken with a fluorescence microscope (Leica Dmi8, Wetzlar, Germany) using a 20X objective. Immunofluorescence analysis revealed amount of MHCII+ cells, which are located next to the neuron in DRG of the TDI-sensitized mice as well as control and their number increased significantly during TDI-induced ACD. (B) The quantification of MHCII+ cells in DRG of control and TDI-sensitized mice. The results were expressed as percentages of MHC+ cells in relation to NeuN+ neurons as mean ± S.D. (N = 8, * p < 0.05). (C) Flow cytometric analysis indicated an increase of percentage of MHCII+ cells in relation to total viable cells in DRG of TDI-sensitized mice comparing to control. Results are expressed as mean ± S.D. (N = 6, * p < 0.05). (D) Representative dot plot of DRG cell suspension from the TDI-sensitized and control group

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BMC Immunology

ISSN: 1471-2172

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