Figure 1

Tumor-bearing mice have an expanded pool of MDSC in spleen and blood. Splenocytes and blood cells from naïve (A-D) and tumor bearing BALB/c (E, F) and C57BL/6 mice (G, H) were stained for CD11b and Gr1 markers and analyzed by flow cytometry as detailed in the Materials and Methods section. Dot plot panels are representative of 8 independent experiments for a total 10 naïve (A, B) and 38 tumor-bearing BALB/c mice (E, F) and 15 naive (C, D) and 15 RMA-bearing C57BL/6 mice (G, H), respectively. Histograms report the percentage of CD11b⁺ Gr1^- and CD11b⁺ Gr1⁺ cells as aggregated data (average ± SD) for the BALB/c (I, J) and the C57BL/6 models (K, L), respectively. Statistic analysis of collected data was performed using the Student's T test; ***p < 0.001, **0.001 < p < 0.05.