Figure 2

MDSC suppress T cells proliferation. MDSC were purified as CD11b⁺ cells from the spleen of C26GM (A, B) and RMA (C) tumor bearing mice by immunomagnetic beads sorting as detailed in the Materials and Methods section. CD11b⁺ cells were added at a final concentration of 20% to a mixed leukocyte culture set up with splenocytes, as responders, stimulated with anti-CD3 and anti-CD28 antibodies (A, C) or with an equal number of γ-irradiated C57BL/6 splenocytes (B). Data are representative of at least two experiments and are expressed as the cpm mean ± SD of triplicates. Student's T test: **0.001 < p < 0.05.