Figure 6

Treatment of tumor-bearing mice with L-NAME does not perturb TDLN cell composition. Cells from TDLN of C26GM- and RMA-bearing mice treated with L-NAME or vehicle (V) and from LN of naïve strain-related mice were either enumerated (A, B) or stained with T cell markers and analyzed by flow cytometry. Histograms report the percentage and absolute number of CD4⁺ (C, E, and D, F), CD8⁺ (G, I, and H, J), and CD4⁺CD25⁺Foxp3⁺ T cells (Terg; K, M, and L, N), respectively, as aggregated data (average ± SD) for the experimental groups reported in the legend of Fig. 3. Student's T test: **0.001 < p < 0.05, *p < 0.05.