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Figure 7 | BMC Immunology

Figure 7

From: Modulators of arginine metabolism support cancer immunosurveillance

Figure 7

Treatments with L-NAME improves IFN-γ release. C57BL/6 mice were challenged with RMA cells and treated with L-NAME (E, F) or vehicle (V; panels C and D). After 15 days, animals were killed and their splenocytes were specifically restimulated in vitro. Blasts were tested for IFN-γ production (upon challenge with RMA or EL4G-) and analyzed by FACScalibur® after co-staining with mAb against CD8, CD44 and IFN-γ. Panel A and B report representative dot plot analyses of blasts from cultures of naïve splenocytes that were challenged with RMA (A) or EL4G- targets (B). The percentage of CD8+IFN-γ+ cells is reported in each panel as average ± SD of at least five animals for experimental group. The same data is reported in histogram G together with the statistical analysis. Student's T test: **0.001 < p < 0.05.

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