Figure 5

Frequency and functional changes in tumor-associated macrophages and microglia (TAMs) in ex vivo tumor specimens over the course of tumor development. While the percentage of TAMs (CD11b⁺CD45⁺ cells) was increased by the final time-point (1.1% vs. 5.6%, P = 0.017), their functional capacity was impaired as measured by TNF-a expression (25.2% vs. 10.9%, P = 0.007). 13 mice were analyzed at 13 dpi (n = 4), 26 dpi (n = 4), and at 29 dpi upon exhibiting clinical tumor morbidity (n = 1) or at 40 dpi (n = 4). Differences between the means at each time point were tested using two-sided t tests with unequal variances. *, P < 0.05; **, P < 0.01, ***, P < 0.001.