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Figure 2 | BMC Immunology

Figure 2

From: Identification of conformational epitopes for human IgG on Chemotaxis inhibitory protein of Staphylococcus aureus

Figure 2

Affinity-purified anti-CHIPS IgG recognize non-linear epitopes. Affinity-purified anti-CHIPS1–121 IgG was tested for the ability to bind CHIPS derived 25-mer peptides or full-length CHIPS in ELISA (A). Full-length CHIPS1–121 or CHIPS with N-terminal truncation (CHIPS ΔN), or CHIPS with truncations in both the N- and C-terminal ends (CHIPS ΔN/C) were compared for reactivity with different human affinity-purified anti-full-length CHIPS1–121 IgG (B), anti-CHIPS ΔN/C IgG (C), and the CHIPS specific mouse monoclonal antibody 2G8 as control (D).

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