Figure 4

Synthetic peptides can be used to purify CHIPS specific antibodies. Binding of affinity-purified anti-CHIPS ΔN/C IgG (a-CHIPS), anti-group 1 peptide (a-Gr 1 pep) and IV-IgG to immobilized CHIPS_1–121 were measured in ELISA (A) and SPR (B). Black bars represent binding of the different affinity-purified antibodies. White bars show binding of antibodies that were pre-incubated with CHIPS_1–121.