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Table 3 Suggested anti-CHIPS ΔN/C IgG epitopes and amino acid substitutions that have been made to verify epitopes*.

From: Identification of conformational epitopes for human IgG on Chemotaxis inhibitory protein of Staphylococcus aureus

Consensus group 1

M

N

K

T/S

x

x

x

Surface 1.1

 

N55K

K100A

T53G

S107N

Y108

 

mutations

 

86%

78%

93%

76%

-

 

Surface 1.2

 

N55K

K100A

S107N

Y108

  

mutations

 

86%

78%

76%

-

  

Surface 1.3

 

Q58K

K100A

S107N

Y108

  

mutations

 

90%

78%

76%

-

  

Surface 1.4

 

N55K

K54R

T53G

Y108

  

mutations

 

86%

90%

93%

-

  

Surface 1.5

 

N68A

K69T

G70

Y71

Y72

 

mutations

 

80%

88%

-

96%

-

 

Surface 1.6

 

N111K

K95S

Y94H

Y97K

Y71K

 

mutations

 

78%

47%

97%

49%

96%

 

Consensus group 2

G

K

L

P

x

x

x

Surface 2.1

 

K69T

L90E

P35A

K92E

E67K

 

mutations

 

88%

92%

97%

85%

91%

 
  1. * The suggested epitopes are denoted Surface 1.1–1.6 and 2.1.
  2. Binding of anti-CHIPS ΔN/C IgG to CHIPS variants was determined by ELISA and is shown as % binding to anti-CHIPS ΔN/C IgG in the table. Nine unique CHIPS variants with a maximum binding lower than 90% of wt CHIPS were found. These are marked in bold and were selected for further characterizations. In some positions no substitutions were made. This is marked by -.