Figure 1From: Tumor necrosis factor-alpha induced expression of matrix metalloproteinase-9 through p21-activated Kinase-1Expression of MMP-9 by human skin of wounds is partially reconstituted by the skin cells. (A) Gelatianses expression by human skin. Biopsies of normal skin, healed and non-healed wounds (full thickness, about 100 mg) were immersed in 1 ml DMEM for 6-hrs and secreted gelatinases were examined by zymography. (B) Early passages of human dermal fibroblasts (HDFs) were cultured on either plastic or in 3D type-I collagen, and treated by cytokines. After 3 days the conditioned medium was examined for gelatinases. (C) The mRNA levels of MMP-9 from HDFs cultured on plastic or in 3D type-I collagen with or without cytokines for 16 hrs were analyzed by real-time RT-PCR. (D) Zymography of the conditioned medium from the primary human keratinocytes (HKC) cultured for 3 days.Back to article page