Figure 3

TNF-α-induced expression of MMP-9 through PAK1, and stabilization of the ectopically expressed kinase by human keratinocytes. Immortalized human keratinocytes transduced by lentivrius expressing GFP or PAK1 variants were cultured in KGM. After culture for 3 days with cytokines the gelatinases in the conditioned medium were measured by zymography. (B) PAK1 and a non-specific protein as a negative control were measured by Western blot analysis.