Figure 7

NF-κB pathway is required for MMP-9 expression, but is independent of PAK1 and p38 MAP kinase. (A) HDFs transduced by lentivrius expressing GFP, PAK1 (K299R), p38 MAP kinase, and its dominant negative ones were cultured on plastic and treated by TNF-α for the indicated intervals. Degradation of I-kappaB was measured by Western blot analysis. (B) HDFs were treated by TNF-α with IKK inhibitors at the indicated concentration for 6 hrs. The mRNA of MMP-9 was measured by real-time RT-PCR analysis. Results are from the average of three experiments. (C) HDFs were treated by TNF-α with IKK inhibitors at low concentration for 3 days. MMP-9 and -2 were measured by zymography.