Figure 2
From: Anti-idiotypic antibodies: a new approach in prion research
The mice immune responses elicited against different antigens. (A) ELISA: immune sera were tested at 10-fold dilution steps starting from a 1:100 dilution. Pre-immune serum was used as the negative control. Each line represents the mean absorbance of 5 individual mice sera ± standard deviation. The antigens used for the immunizations are listed in the upper right corner. (B) Competition assay: V5B2 was pre-incubated with the serially diluted sera of mice immunized with different forms of V5B2 (listed in the upper right corner) and the V5B2 binding to the P1 peptide was measured by ELISA. Each column represents the mean absorbance of 4 individual mice sera with the highest antibody titres ± standard deviation.