Skip to main content
Figure 4 | BMC Immunology

Figure 4

From: Anti-idiotypic antibodies: a new approach in prion research

Figure 4

Competitive immunohistochemical studies. (A) Representative photomicrographs of immunohistochemically stained sCJD cerebellar cortex sections in the absence and presence of different competitor molecules. The brown precipitate in the Purkinje cells layer and internal granular cell layer indicates specific prion immunoreactivity to a variable degree in all of the images except the negative control. Only photomicrographs of sections stained with the use of following concentrations of competitor molecules are shown: 100 μg/ml for isotype control, 5D12 and 4F8, and 10 μg/ml for Ch5, P1 and Pscr. (B) Bar chart showing the effects of different competitor molecules at varying concentrations on the mean immunohistochemical signal intensity, expressed as percentages of the uncompeted V5B2-HRP immunoreactive signal intensity (positive control). Asterisks indicate statistically significant (p < 0.004) reductions in the mean signals. Competitor molecule concentrations are given in μg/ml in brackets.

Back to article page