Figure 7

Nrg necessary for proper encapsulation of L. boulardi eggs (A) Encapsulation capacities of Nrg mutant larvae in response to parasitisation by L. boulardi G486. [(Number of larvae with properly melanized wasp eggs/number of parasitized larvae) × 100)]. Numbers above the bars indicate the number of wasp parasitized larvae examined, numbers in parentheses indicate percentage of larvae that properly encapsulated the L. boulardi G486 eggs (B, C) Encapsulated wasp eggs recovered from larvae 22–24 h after parasitisation, and stained with the plasmatocyte specific protein Nimrod. (B) Control (w¹¹¹⁸) and (C) Nrg^{G 00305}homozygous mutants. (D, E) Haemocytes 38–40 h post-parasitisation stained with the L1 lamellocyte specific antibody. (D) Control and (E) Nrg^{G 00305}homozygous mutants. (F) Control and Nrg^{G 00305}plasmatocytes attached to L. boulardi G486 eggs. Unlike controls, plasmatocytes from Nrg^{G 00305}extend filopodia from their apical side.