|  | Illumina array fold change | Real time PCR | |||||
---|---|---|---|---|---|---|---|---|
 |  |  |  |  | Ct |  | RQ fold * |  |
Ref.Seq | Gene Symbol | PMA24 | LPS | VitD3 | Cont | PMA24 | LPS | VitD3 |
Cytokines and Chemokines | ||||||||
NM_002983 | CCL3 | 7.1 | 197.3 | 1.4 | 26 | 27.9 | 776 | 1.6 |
NM_000594 | TNFa | 3 | 121.4 | 1.6 | 29 | 13.9 | 119.4 | 1.1 |
NM_000584 | IL8 | 3.7 | 82.9 | 2.3 | 25 | 1.2 | 222.9 | 3.2 |
NM_000575 | IL1A | 1.1 | 98.7 | -1 | 35 | 5.7 | 2195 | 2.3 |
NM_000576 | IL1B | 2.9 | 75.2 | 1.1 | 26 | 2.5 | 415.9 | 0.7 |
NM_004591 | CCL20 | -1 | 47.6 | 1.1 | 30 | 2.1 | 724.1 | 0.9 |
NM_000600 | IL6 | 3.7 | 29.9 | 2.1 | 28 | 4.6 | 157.6 | 3.5 |
NM_001565 | IP10 | 7.5 | 23.4 | -1 | 30 | 97 | 222.9 | 0.7 |
NM_002089 | CXCL2 | 1.2 | 16.5 | 1.1 | 28 | 0.4 | 48.5 | 1.7 |
NM_002984 | CCL4 | 1.1 | 1.7 | 1.1 | 35 | 52 | 80,000 | 1.9 |
Surface molecules | ||||||||
NM_004233 | CD83 | 1.8 | 10.4 | -1.1 | 23 | 3.2 | 9.8 | 0.6 |
NM_001781 | CD69 | 1.4 | 4.1 | -1.4 | 21 | 3.2 | 4.9 | 0.9 |
NM_000632 | CD11b | 3.3 | 3.1 | 2.7 | 24 | 7.5 | 3 | 2.6 |
NM_000591 | CD14 | -1 | 1.2 | 22.4 | 29 | 1.1 | 1.1 | 45.3 |
Signaling and effector molecules | ||||||||
NM_002423 | MMP7 | 29.9 | 36.5 | -1.4 | 28 | 512 | 181 | 0.6 |
NM_002658 | PLAU | 1.3 | 19.9 | -1 | 28 | 1.1 | 5.3 | 0.6 |
NM_000265 | NCF1 | 18.6 | 17.8 | 3.8 | 29 | 32 | 39.4 | 2.6 |
NM_005627 | SGK | 7.5 | 13 | -1 | 22 | 10.6 | 52 | 1.6 |
NM_000389 | p21 | 6.1 | 11.8 | 1.3 | 25 | 42.2 | 42.2 | 1.1 |
NM_000014 | A2M | 10.4 | 11.5 | 1.1 | 30 | 52 | 32 | 3.2 |
NM_004994 | MMP9 | 8.4 | 8.3 | -1.2 | 30 | 21.1 | 9.2 | 1.1 |
NM_000228 | LAMB3 | 2.9 | 6.5 | 8.5 | 28 | 32 | 19.7 | 64 |
NM_002468 | MYD88 | 4.5 | 4.2 | 1.4 | 27 | 27.9 | 7.5 | 1.5 |
NM_000064 | C3 | 1.8 | 2.3 | -1 | 27 | 119.4 | 42.2 | 1.2 |
NM_003264 | TLR2 | 1.4 | 1.6 | 1.1 | 31 | 11.3 | 3.2 | 2 |
NM_000215 | JAK3 | 1.5 | 1.7 | 1.1 | 18 | 5.7 | 4.9 | 1.4 |
NM_004345 | LL37 | -1.2 | -1.3 | 32.1 | 30 | 1.3 | 0.8 | 84.4 |
NM_001775 | CD38 | -1.9 | -2 | 5.9 | 22 | 1.1 | 0.2 | 7.5 |
NM_001911 | CTSG | -3.4 | -4.8 | -1.3 | 21 | 0.03 | 0.01 | 0.6 |
NM_006573 | TNFSF13B | -4.3 | -5.1 | -1.2 | 22 | 0.3 | 0.1 | 1.9 |
NM_000250 | MPO | -8.3 | -9.6 | -1.1 | 17 | 0.1 | 0.03 | 1.1 |
Transcription factors/Histone modifiers | ||||||||
NM_005384 | NFIL3 | 3.1 | 4.9 | -1.3 | 27 | 9.8 | 78.8 | 3.2 |
NM_000321 | RB1 | 2.2 | 1.8 | 1 | 19 | 3 | 1.1 | -0.2 |
NM_005461 | MAFB | 3.3 | 35.8 | 1.2 | 27 | 17.1 | 18.4 | -1.1 |
NM_005587 | MEF2A | 4 | 4.4 | 1.1 | 19 | 9.2 | 4.3 | 1.1 |
NM_002397 | MEF2C | 1.8 | 1.9 | -1.2 | 19 | 8 | 4.3 | 1.6 |
NM_005920 | MEF2D | 2.1 | 7.9 | -1.1 | 26 | 1.1 | 3 | 0.5 |
NM_021958 | HLX1 | 2.2 | 2.3 | -1 | 23 | 10.6 | 3.5 | 1.2 |
NM_021999 | BRI | 2.6 | 2.2 | -1.2 | 22 | 8.6 | 3.7 | 1.2 |
NM_005474 | HDAC5 | NA | Â | Â | 24 | 4.9 | 4 | 0.8 |
NM_012412 | H2AV | NA | Â | Â | 19 | 2.6 | 1 | 1.3 |
NM_173158 | Nur77 | 1.2 | 11.7 | 1.1 | 35 | 1.1 | 2.3 | 1 |
NM_001706 | BCL6 | 7.6 | 19.5 | 1.6 | 25 | 78.8 | 34.3 | 8 |
NM_001964 | EGR1 | 4.2 | 13.6 | 1.1 | 27 | 14.9 | 1.2 | 1.4 |
NM_005252 | FOS | 2.4 | 3.3 | -1.5 | 26 | 32 | 2.6 | 0.9 |
NM_002167 | ID3 | 11.9 | 38.9 | 2.7 | 30 | 13 | 18.4 | 8 |
NM_006084 | IRF9 | 2.5 | 2.8 | 1 | 25 | 214.5 | 5.3 | 1.4 |
NM_000399 | EGR2 | 5.3 | 27.3 | 1.1 | 28 | 5.3 | 4.3 | 2.5 |
NM_016270 | KLF2 | 13.9 | 9.8 | -1.3 | 26 | 32 | 97 | 0.5 |
NM_021784 | FOXA2 | 4.9 | 2.9 | -1 | 30 | 73.5 | 16 | 0.3 |
NM_005524 | HES1 | 2.8 | 2.8 | 1.2 | 32 | 27.9 | 64 | 0.8 |
NM_004235 | KLF4 | 1.5 | 1.7 | -1 | 27 | 3.7 | 3 | 1.9 |
NM_002698 | OCT2 | 2.1 | 3.3 | -1.2 | 26 | 14.9 | 7 | 0.4 |
NM_030756 | TCF7L2 | 3 | 2.8 | -1.2 | 21 | 8.6 | 5.3 | 0.5 |
NM_004030 | IRF7 | 2.2 | 2.6 | -1.3 | 26 | 13 | 4.6 | 1.2 |
NM_014707 | HDAC7 | 3.2 | 2.5 | -1.2 | 22 | 29.9 | 16 | 0.7 |
NM_003884 | PCAF | 2 | 1.8 | -1.2 | 25 | 3.7 | 1.4 | 0.8 |
NM_005919 | MEF2B | 1.3 | 1.4 | 1.3 | 27 | 6.5 | 0.4 | 0.8 |