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Table 3 Cross validation of 24 transcriptional regulators by real-time PCR

From: Identification of novel transcriptional regulators involved in macrophage differentiation and activation in U937 cells

  

Ct

ddCt

 

Ct

ddCt

  

Ct

ddCt

 

Ref.Seq

gene

Cont

PMA24

LPS

Mono*

Macro*

LPS*

 

Mono*

Macro*

LPS*

NM_005587

MEF2A

19.1

-3.2

-2.1

25.2

-2.4

-3

≥

21.2

-1

-1.2

NM_002397

MEF2C

18.9

-3

-2.1

25.5

-2.8

-3

≥

19.8

-1.8

-1.8

NM_005920

MEF2D

25.5

-2.4

-1.6

26.4

-0.7

-1

≈

27.7

-0.8

-1.5

NM_021958

HLX1

22.6

-3.4

-1.8

24.7

-2.1

-2

≈

24.7

-2.2

-2.6

NM_021999

BRI

22.2

-3.1

-1.9

26

-2.9

-3

≈

18.9

-2

-2.8

NM_005474

HDAC5

23.7

-2.3

-2.2

26.8

-2

-2

≈

25.5

-1.8

-2

NM_012412

H2AV

18.6

-1.4

0.1

25.7

-1.1

-2

≈

19

-0.9

-1.3

NM_173158

Nur77

35

-0.2

-1.2

33.4

0

-2

≈

33.5

-1.7

-1.2

NM_001706

BCL6

24.6

-6.3

-5.1

27.8

0.3

-1

≈

20

-0.3

-1.3

NM_001964

EGR1

26.8

-3.9

-0.3

30.5

0.4

-5

≥

22.7

8.1

-2.7

NM_005252

FOS

25.5

-5

-1.4

23.7

2.8

-1

≈

21.6

2.7

-2

NM_002167

ID3

29.6

-3.7

-4.2

31

1.2

1.1

≠

23.5

-2.8

-2.3

NM_006084

IRF9

25.1

-4.5

-2.4

23.9

-1.6

-1

≠

21.9

0.3

-0.1

NM_000399

EGR2

28.2

-2.4

-2.1

27.7

2.6

0

≤

23.8

4.4

-0.7

NM_016270

KLF2

26.4

-5

-6.6

21.7

3

2.4

≈

16.7

5.1

3

NM_021784

FOXA2

29.5

-6.2

-3.7

30.2

3.3

2.2

≈

32.3

3

3

NM_005524

HES1

31.5

-4.8

-6

28

6.9

2.7

≈

21.5

11

9

NM_004235

KLF4

26.6

-3.9

-1.6

22

6.5

3.9

≈

20.3

4.9

4

NM_002698

OCT2

25.5

-3.9

-2.8

22.5

-0.5

-1

≠

19.4

0.8

1

NM_030756

TCF7L2

20.7

-3.1

-2.4

25

-0.4

0

≠

20.7

1.8

1

NM_004030

IRF7

26.4

-3.7

-2.2

25.3

0.1

-1

≠

23.9

1.6

1

NM_014707

HDAC7

22.2

-4.9

-4.2

27.8

1.4

1.1

≠

22.1

-0.3

-0.7

NM_003884

PCAF

25.1

-1.9

1.5

28.1

0.7

1.2

≠

28.4

-0.6

-0.9

NM_005919

MEF2B

27.3

-2.7

1.4

31.9

-0.3

0

≠

27.6

-0.5

-1.3

     

Primary cells

     
  

U937 cells

 

donor 1

   

donor 2

 
  1. * Primary monocytes were isolated from the blood of two healthy individuals designated as donor 1 and donor 2, differentiated by M-CSF into macrophages and stimulated by 10 μg/ml LPS. Degree of individual heterogeneity between two donors was expressed as nearly equal (≈), equal with different propensity (≥ or ≤), and opposite (≠).
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BMC Immunology

ISSN: 1471-2172

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