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Table 3 Cross validation of 24 transcriptional regulators by real-time PCR

From: Identification of novel transcriptional regulators involved in macrophage differentiation and activation in U937 cells

   Ct ddCt   Ct ddCt    Ct ddCt  
Ref.Seq gene Cont PMA24 LPS Mono* Macro* LPS*   Mono* Macro* LPS*
NM_005587 MEF2A 19.1 -3.2 -2.1 25.2 -2.4 -3 21.2 -1 -1.2
NM_002397 MEF2C 18.9 -3 -2.1 25.5 -2.8 -3 19.8 -1.8 -1.8
NM_005920 MEF2D 25.5 -2.4 -1.6 26.4 -0.7 -1 27.7 -0.8 -1.5
NM_021958 HLX1 22.6 -3.4 -1.8 24.7 -2.1 -2 24.7 -2.2 -2.6
NM_021999 BRI 22.2 -3.1 -1.9 26 -2.9 -3 18.9 -2 -2.8
NM_005474 HDAC5 23.7 -2.3 -2.2 26.8 -2 -2 25.5 -1.8 -2
NM_012412 H2AV 18.6 -1.4 0.1 25.7 -1.1 -2 19 -0.9 -1.3
NM_173158 Nur77 35 -0.2 -1.2 33.4 0 -2 33.5 -1.7 -1.2
NM_001706 BCL6 24.6 -6.3 -5.1 27.8 0.3 -1 20 -0.3 -1.3
NM_001964 EGR1 26.8 -3.9 -0.3 30.5 0.4 -5 22.7 8.1 -2.7
NM_005252 FOS 25.5 -5 -1.4 23.7 2.8 -1 21.6 2.7 -2
NM_002167 ID3 29.6 -3.7 -4.2 31 1.2 1.1 23.5 -2.8 -2.3
NM_006084 IRF9 25.1 -4.5 -2.4 23.9 -1.6 -1 21.9 0.3 -0.1
NM_000399 EGR2 28.2 -2.4 -2.1 27.7 2.6 0 23.8 4.4 -0.7
NM_016270 KLF2 26.4 -5 -6.6 21.7 3 2.4 16.7 5.1 3
NM_021784 FOXA2 29.5 -6.2 -3.7 30.2 3.3 2.2 32.3 3 3
NM_005524 HES1 31.5 -4.8 -6 28 6.9 2.7 21.5 11 9
NM_004235 KLF4 26.6 -3.9 -1.6 22 6.5 3.9 20.3 4.9 4
NM_002698 OCT2 25.5 -3.9 -2.8 22.5 -0.5 -1 19.4 0.8 1
NM_030756 TCF7L2 20.7 -3.1 -2.4 25 -0.4 0 20.7 1.8 1
NM_004030 IRF7 26.4 -3.7 -2.2 25.3 0.1 -1 23.9 1.6 1
NM_014707 HDAC7 22.2 -4.9 -4.2 27.8 1.4 1.1 22.1 -0.3 -0.7
NM_003884 PCAF 25.1 -1.9 1.5 28.1 0.7 1.2 28.4 -0.6 -0.9
NM_005919 MEF2B 27.3 -2.7 1.4 31.9 -0.3 0 27.6 -0.5 -1.3
      Primary cells      
   U937 cells   donor 1     donor 2  
  1. * Primary monocytes were isolated from the blood of two healthy individuals designated as donor 1 and donor 2, differentiated by M-CSF into macrophages and stimulated by 10 μg/ml LPS. Degree of individual heterogeneity between two donors was expressed as nearly equal (≈), equal with different propensity (≥ or ≤), and opposite (≠).