Genetic ablation or pharmacological blockade of dipeptidyl peptidase IV does not impact T cell-dependent immune responses

Table 2 Des-flurositagliptin dosed at ~400 mpk in feed is able to inhibit the DPP IV enzyme activity for 24 hours in blood.

	Conversion of substrate in nmol/min
	Day 1	Day 2	Day 3
Des-flurositagliptin, am bleed	4.53 ± 0.22	5.44 ± 0.79	6.12 ± 0.48
Des-flurositagliptin, pm bleed	4.90 ± 0.37	8.08 ± 1.8	8.29 ± 1.42
Control, am bleed	39.8 ± 4.0	47.5 ± 5.23	40.1 ± 6.94
Control, pm bleed	31.4 ± 0.65	43.3 ± 6.01	40.3 ± 7.65
DPP-IV^-/- mice	1.02 ± 0.51

The animals were fed chow milled with 6.7 grams of desflurositagliptin in 1 kg of Tekland chow to deliver an effective dose of 400 mpk. Blood were drawn 12 hours apart and DPP IV enzyme activity measured as described earlier. The animals (n = 4 in each group) were maintained on the inhibitor for the entire period of the experiment.

ISSN: 1471-2172