Figure 6

Biosynthesis of LTB ₄ by B-PLL cells and MCL lines. Intact cells (10 × 10⁶) were pre-incubated for two min at 37°C, in the presence of Diamide (100 μM), prior to addition of A23187 (1 μM) plus arachidonic acid (AA) (40 μM) for five min. Sonicated cells (homogenate) were pre-incubated with ATP (1 mM) for two min at 37°C and then incubated with calcium chloride (2 mM) and arachidonic acid (40 μM) for five min. Panel A depicts the formation of LTB₄ in B-PLL cells isolated from four different donors (mean ± SD). Panel B depicts the formation of LTB₄ in three different MCL lines i.e. Granta 519, JEKO-1 and Rec1 (mean ± SD, from three independent experiments).