Table 2 List of primers that were used in the RT-PCR experiment
From: High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
Gene | Annealing temp. | Size of PCR Product | sequence |
|---|---|---|---|
5-LO | 72°C | 486 bp | 5'ACCATTGAGCAGATCGTGGACACGC 3'GCAGTCCTGCTCTGTGTAGAATGGG |
FLAP | 71°C | 352 bp | 5'GGCCATCGTCACCCTCATCAGCG 3'GCCAGCAACGGACATGAGGAACAGG |
LTA4H | 72°C | 464 bp | 5'GCAGTCACGGGATGCATGCTTGCT 3'GCCTGGCTCTACTCTCCTGGACTG |
LTC4S | 68°C | 252 bp | 5'TGCCACCACACCGACGGTACCATG 3'CCCTTCATGAAAGAAGATGCCGG |