Skip to main content

Table 1 The effect of metabolic inhibitors on TM-N49 induced histamine release from human mast cells

From: Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2

Compound

(μg/ml)

Net histamine release (%)

  

Without metabolic inhibitors

With metabolic inhibitors

  

Lung

Colon

Tonsil

Lung

Colon

Tonsil

TM-N49

3.0

9.3 ± 1.9

13.5 ± 3.0

8.0 ± 2.0

0.4 ± 0.4*

0.4 ± 0.7*

0.8 ± 0.6*

 

30

14.7 ± 4.7

29.6 ± 6.8

62.0 ± 8.5

1.6 ± 0.9*

0.4 ± 1.4*

49.3 ± 7.5

Anti-IgE

10

17.3 ± 5.4

17.4 ± 5.1

11.1 ± 3.4

0.7 ± 1.7*

0.1 ± 0.8*

2.2 ± 1.5*

CI

0.5

24.4 ± 6.3

38.8 ± 4.6

37 ± 9.3

5.3 ± 5.5*

2.1 ± 0.7*

6.4 ± 2.9*

  1. The values shown are mean ± SE for four to five separate experiments performed in duplicate. Cells were incubated with metabolic inhibitors 2-deoxy-D-glucose (10 mM) and antimycin A (1 μM) for 40 min at 37°C before challenging with stimulus. * P < 0.05 compared with the corresponding uninhibited controls.