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Table 1 The effect of metabolic inhibitors on TM-N49 induced histamine release from human mast cells

From: Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2

Compound
(μg/ml)
Net histamine release (%)
   Without metabolic inhibitors With metabolic inhibitors
   Lung Colon Tonsil Lung Colon Tonsil
TM-N49 3.0 9.3 ± 1.9 13.5 ± 3.0 8.0 ± 2.0 0.4 ± 0.4* 0.4 ± 0.7* 0.8 ± 0.6*
  30 14.7 ± 4.7 29.6 ± 6.8 62.0 ± 8.5 1.6 ± 0.9* 0.4 ± 1.4* 49.3 ± 7.5
Anti-IgE 10 17.3 ± 5.4 17.4 ± 5.1 11.1 ± 3.4 0.7 ± 1.7* 0.1 ± 0.8* 2.2 ± 1.5*
CI 0.5 24.4 ± 6.3 38.8 ± 4.6 37 ± 9.3 5.3 ± 5.5* 2.1 ± 0.7* 6.4 ± 2.9*
  1. The values shown are mean ± SE for four to five separate experiments performed in duplicate. Cells were incubated with metabolic inhibitors 2-deoxy-D-glucose (10 mM) and antimycin A (1 μM) for 40 min at 37°C before challenging with stimulus. * P < 0.05 compared with the corresponding uninhibited controls.