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Table 2 The effect of pertussis toxin on TM-N49 induced histamine release from human mast cells

From: Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2

Compound
(μg/ml)
Net histamine release (%)
   Without pertussis toxin With pertussis toxin
   Lung Colon Tonsil Lung Colon Tonsil
TM-N49 3.0 9.3 ± 1.9 13.5 ± 3.0 8.0 ± 2.0 0.8 ± 1.8* 0.1 ± 2.2* 1.5 ± 1.3*
  30 14.7 ± 4.7 29.6 ± 6.8 62.0 ± 8.5 4.0 ± 0.6* 3.9 ± 3.9* 31.9 ± 9.2*
Anti-IgE 10 17.3 ± 5.4 17.4 ± 5.1 11.1 ± 3.4 2.1 ± 1.1* 0.9 ± 1.2* 3.0 ± 0.3*
CI 0.5 24.4 ± 6.3 38.8 ± 4.6 37 ± 9.3 19.6 ± 4.8 30.4 ± 2.8 20.4 ± 4.4
  1. The values shown are mean ± SE for four to five separate experiments performed in duplicate. Cells were incubated with1.0 μg/ml pertussis toxin for 4 h at 37°C before challenging with stimulus. * P < 0.05 compared with the corresponding uninhibited controls.