Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2

BMC Immunology

Table 3 Effect of TM-N49 on relative mast cell numbers in mouse peritoneum following injection.

TM-N49 (μg)	Percentage of mast cells in total nucleated cells
	10 min	2 h	6 h	16 h
Saline	0.49(0.40–0.96)	0.45(0.31–1.30)	0.56(0.23–0.76)	0.85(0.29–1.88)
50	0.16(0.06–0.35)*	0.24(0.08–0.78)*	0.36(0.21–0.90)	0.05(0.03–0.11)*
5.0	0.76 (0.44–1.11)	1.05(0.60–1.77)*	0.67(0.29–1.76)	0.51 (0.22–1.36)
0.5	n.d	n.d	0.61(0.39–1.00)	0.63 (0.27–1.10)
0.05	n.d	n.d	0.90(0.39–2.67)	1.10 (0.47–1.31)

Various doses of TM-N49 were injected into the peritoneum of mice for 10 min, 2 h, 6 h or 16 h. Also shown are the responses to normal saline control. Relative number of mast cells was defined as the percentage of mast cell in total nucleated cells. * P < 0.05 compared with the response to saline alone.

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